Enzymes and Inhibitors
Biotium Coelenterazine FCP
Coelenterazine fcp is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is 135 times higher than that of aequorin formed from native coelenterazine. Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C25H24FN3O2; MW: 417.48.
Abcam Ceefourin™ 1, MRP4 inhibitor, 1MG
MW 294.4 Da, Purity >99%. Highly selective multidrug resistance protein 4 (MRP4) inhibitor (IC50 = 1.5 µM). Blocked transport of MRP4 substrates 6-Mercaptopurine, SN 38, cAMP, D-Luciferin and E217βG. No detectable effect on MRP4 homologs MRP1, 2, 3 or 5, and related transporters P-gp and ABCG2 *in vitro*. Low cellular toxicity, and high microsomal and acid stability. Chemically distinctive to Ceefourin™ 2 (ab145145).
The product is subject to the following: Abcam Restricted Use Statement
Ubpbio Ub-AMC, 50UG
Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
Ubpbio A fluorogenic substrate of the caspase-like activity of proteasomes.
Z-LLE-AMC is a fluorogenic substrate for the peptidylglutamyl peptide hydrolyzing (PGPH) activity or the caspase-like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Sino Biological Peptide Substrates: Micro2 Peptide
The Micro2 Peptide sequence (KEEQSQITSQVTGQIGWR) is derived from human AP-2 complex subunit mu isoform b (amino acids 143-160) and is suitable as a substrate for AAK1, BMP2K, and GAK kinase assay.
Research Products International Corp D-Luciferin Sodium Salt 10 G
RPI offers the highest purity D-Luciferin Sodium Salt for a wide range of bioluminescent applications. Allows highly sensitive bioluminescent measurements. Ideal for reporter gene assays, ATP assays, whole animal studied, DNA sequencing and more. Our product is specially purified and tested extensively to ensure the highest signal and reproducibility with low background and sensitivity. Guaranteed purity is determined by both standard HPLC and Chiral HPLC methods. Protect from light and moisture.
Sino Biological SwiftFluo TR-FRET BTKs substrate - biotin 25 nmol
SwiftFluo TR-FRET BTKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with SwiftFluor™ TR-FRET TKs kinase kit (Cat. # SFKA001)
Sino Biological SwiftFluo TR-FRET FGFRs substrate - biotin 25 nmol
SwiftFluo TR-FRET FGFRs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with SwiftFluo TR-FRET TKs kinase kit (Cat. # SFKA001).
AnaSpec 520 MMP FRET Substrate I, Sensitive FRET Assay Tool, High Purity ≥95%, 0.1 mg
The 520 MMP FRET Substrate I is a sensitive fluorogenic peptide specially designed for detecting Matrix Metalloproteinases (MMPs). Its unique FRET mechanism allows rapid enzymatic activity measurements, particularly for MMP-13. This substrate excels in research areas like cancer and apoptosis, offering a reliable way to quantify protease activity with clear fluorescent readouts, differentiate enzymatic activity, and ensure reliable results for scientific studies.
Medchemexpress LLC Z-DEVD-AMC | 98.2% | 767.74 | 10 MG
Z-DEVD-AMC is a selective caspase-3 substrate that can be measured by fluorescence spectrometry. It serves as a fluorescence reference standard for AMC-based enzyme substrates, including AMC-based caspase substrates.
- Selective caspase-3 substrate
- Measured by fluorescence spectrometry
- Functions as a fluorescence reference standard for AMC-based enzyme substrates
- Purity of 98.2%
- Molecular weight of 767.74
- White to off-white solid appearance
- Research use only
- Excitation wavelength of 390
- Emission wavelength of 480
Ubpbio K48-linked tri-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Seracare Life Sciences Inc TrueBlue™ Peroxidase Substrate 200ml
TrueBlue is up to 100 times more sensitive than DAB and it is free of known carcinogens. Strong blue reaction product provides excellent contrast with DAB and other substrates. HistoMark TrueBlue provides the most sensitive means available to detect peroxidase-labeled conjugates. Because of its high sensitivity, TrueBlue is recommended for detection of the least abundant marker. TrueBlue is alcohol and xylene insoluble.
Medchemexpress LLC HY-111382 10mg Medchemexpress, Diphenylterazine CAS:344940-63-2 Purity:>98%
Medchemexpress, HY-111382 10mg Diphenylterazine CAS:344940-63-2 Diphenylterazine is a bioluminescence agent. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.